Biotinylated Human PCSK9 Protein, Avitag™,His Tag (MALS verified)

Description	Biotinylated Human PCSK9, Avitag,His Tag (PC9-H82E7) is expressed from human HEK293 cells. It contains AA Gln 31 - Gln 692 (Accession # Q8NBP7-1). Proprotein convertase subtilisin/kexin type 9 (PCSK9) is also known as NARC1 (neural apoptosis regulated convertase), is a newly identified subtilase belonging to the peptidase S8 subfamily. Mouse PCSK9 is synthesized as a soluble zymogen, and undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted active enzyme with a broad alkaline pH optimum. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3). This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. This protein undergoes autocatalytic cleavage to release the pro-peptide and mature chain. The pro-peptide and mature chain are associated through non‑covalent interactions and with a calculated MW of 13.8 kDa and 59.9 kDa respectively. The protein migrates as 17 kDa and 63-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Conjugate	Biotin
Weblink	https://www.acrobiosystems.com/P1427-Biotinylated-Human-PCSK9%A0Protein%A0Avitag%E2%84%A2His%A0Tag-%28MALS-verified%29.html
Datasheet	https://www.acrobiosystems.com/static/main/products/pdf/ds/PC9-H82E7.pdf
Transportation	RT
Storage	-20°C